The generally used MTS-based assay is compared with a bioassay employing a water-soluble tetrazolium dye, WST-8, using NFS-60 cells at a concentration of 7×10⁵?cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay is used to test the potency of various commercially available PEGylated G-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

601.48

C₂₀H₁₃N₆NaO₁₁S₂

193149-74-5

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

In Vitro:?

H₂O : 83.33 mg/mL?(138.54 mM;?Need ultrasonic)

DMSO : 10 mg/mL?(16.63 mM;?Need ultrasonic)

配制儲備液

濃度溶劑體積質量1 mg5 mg10 mg1 mM1.6626 mL8.3128 mL16.6257 mL5 mM0.3325 mL1.6626 mL3.3251 mL10 mM0.1663 mL0.8313 mL1.6626 mL

*

請根據產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 儲存時，請在 6 個月內使用，-20°C 儲存時，請在 1 個月內使用。

In Vivo:

請根據您的實驗動物和給藥方式選擇適當的溶解方案。以下溶解方案都請先按照?In Vitro?方式配制澄清的儲備液，再依次添加助溶劑：

——為保證實驗結果的可靠性，澄清的儲備液可以根據儲存條件，適當保存；體內實驗的工作液，建議您現用現配，當天使用； 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現沉淀、析出現象，可以通過加熱和/或超聲的方式助溶

• 1.

  
  

  請依序添加每種溶劑：?PBS

  Solubility: 100 mg/mL (166.26 mM); Clear solution; Need ultrasonic

  
  

• 2.

  
  

  請依序添加每種溶劑：?10% DMSO ?? 40%?PEG300??? 5%?Tween-80??? 45% saline

  Solubility: ≥ 1 mg/mL (1.66 mM); Clear solution

  
  

• 3.

  
  

  請依序添加每種溶劑：?10% DMSO ?? 90% (20%?SBE-β-CD?in saline)

  Solubility: ≥ 1 mg/mL (1.66 mM); Clear solution

  
  

• 4.

  
  

  請依序添加每種溶劑：?10% DMSO ?? 90%?corn oil

  Solubility: ≥ 1 mg/mL (1.66 mM); Clear solution